Subject:
NEW DISCOVERY: QO4 / "QUAD-ATOMIC OXYGEN"
CONGRATULATIONS BERKANA
AS BEING "STATE OF THE ART"
PRESS RELEASE: October 18, 1999 New Oxygen Molecule Discovered
San Luis Obispo,
CA: BIO2 International, Inc., and Birmingham, AL: Connections
Group, Inc. the exclusive distributor of the premier stabilized
oxygen solution JozzerÒ Hyper-Oxygen Drops also known
as ASOÒ both in 25% & 35% strengths and as just announced
the results of a research study that confirms the existence
of a new molecule of oxygen within ASOÒ which the researchers
have named "quad-atomic oxygen™", or QO4™.
Activated Stabilized Oxygen ASOÒ, which is based upon
dissolved molecular oxygen rather than the salts of oxygen (NaClO2)
formulations. The amount of dissolved oxygen in water is usually
small (7 - 20 parts per million). Nevertheless, water is capable
of holding a greater concentration of molecular oxygen.
The purpose of this
research, which was reported by Third State Analytical on 10/5/99,
was to test the theory that ASOÒ might contain a new
molecular form of oxygen, primarily QO4™. The existence
of QO4™, which is theoretically comprised of 4 atoms of
oxygen, had never been proved or quantified prior to this research.
Oxygen is essential to the life and health of every one of the
75 trillion cells in our body. Under the influence of various
enzymes, oxygen acts with ingested carbohydrates, fats and proteins
to form ATP, the high energy storage compound that is used to
energize almost every ntracellular metabolic function and reaction,
within the mitochondria of the cells.
Conventional oxygen
testing procedures have been inconsistent and inconclusive in
determining the total free oxygen concentration in ASOÒ.
Moreover, none of these standard test procedures could identify
all of the molecular free oxygen molecules in ASOÒ. It
was theorized that ASOÒs unique characteristics of being
highly stable in solution as well as a potent anti-microbial
agent might be explained through understanding its component
structure; that is, the type or types of oxygen present in JozzerÒ
Hyper-Oxygen ASO.Ò Other oxygen molecules have been theorized
(O4, O8, O16). However, until now, no research had ever been
done to confirm or deny the existence of such molecules and
their relative stability in an aqueous (water) medium. The researchers
for this study believed that if such molecules of oxygen did
exist, then the relative molecular stability of such molecules
would most likely be greater than their O2 counterparts, perhaps
as much as four times more stable. This could explain, if QO4™
were found to exist, why it might be considerably more stable
in an aqueous solution.
Researchers tested
the ASOÒ using state-of-the-art molecular analysis equipment
and newly developed computer analytical software. Using these
tools, the researchers tested the theory at several universities
for statistical consistency. The primary analytical instrument
used in this research was the Perkins Elmer ELAN® 6000 ICP-Mass
Spectrometer. The ELAN® 6000 can assess a sample, covering
the full range of the elements in the periodic table, in less
than two minutes. The ELAN® 6000 was initially developed
to accurately measure at parts-per-trillion (PPT) detection
limits. Utilizing this ultra sensitive instrument, the research
conducted confirmed the presence of O1, O2, & QO4™
at a concentration of <0.00003 mg/L, <0.00025mg/L, and
0.23567 mg/L respectively. In other words, AO2C™ was found
to contain over 940 times as much QO4™ as O2!
The conclusions of
this study are the following:
· JozzerÒ
Hyper Oxygen ASOÒ contains a unique, previously undiscovered,
molecule of oxygen containing four atoms of oxygen in a relatively
stable configuration called "quad-atomic oxygen™",
or QO4™.
· The presence
of a significant amount of QO4™ represents a free oxygen
concentration in ASOÒ greater than 23% total oxygen by
volume in the solution. (For point of reference, hydrogen peroxide
solution commonly used as a disinfectant and antiseptic contains
3% oxygen in the form of O1).
· QO4™
represents a form of oxygen that reacts quite differently than
other forms of oxygen due to its apparent concentrated stability
in an aqueous (water) solution as well as its apparent non-toxic
properties.
· QO4™
may be the primary molecule responsible for ASOÒ's anti-microbial
disinfectant properties.
It
is the researchers' opinion that the oxygen molecules in JozzerÒ
Hyper Oxygen ASOÒ may represent a new paradigm in reactive
yet stable and safe oxygen for use in human physiology as well
as antiseptic and disinfectant applications, in a wide spectrum
of diverse industries. While the full scope and impact of the
properties of QO4™ are as yet undefined, the initial human
metabolic research and pathogenic microorganism anti-microbial
data indicates that JozzerÒ Hyper Oxygen ASOÒ
has great potential and promise to reduce disease and enhance
the health of individuals throughout the world.
Note: Berkana Oxygen-Rich product line only uses the highest
concentrations of ASOÒ at 25% * 35% strengths- known
as JozzerÒ Hyper Oxygen Drops maximum and professional
strength’s.
CERTIFICATE OF ANALYSIS MANUFACTURING INGREDIENTS And PRODUCT
STABILITY
This is to certify
that the product
ASO® or AO2®, or Activated Oxygen
Super-Concentrated
(SC) 35% Jozzer Hyper Oxygen Drops
Contains the following
ingredients (by percentage of total volume):
Distilled Water (62.04%)
CAS 7732-18.5
Molecules of ASO®
Activated Stabilized Oxygen (35.0%)
Dissolved Trace Minerals
and Sodium Chloride (2.96%) CAS 7647-14-5
Other Analytical
Specifications:
Specific Gravity:
1.016 23º/23º C
Density: 1.016 g/cm3
pH: 8.46
Shelf Life/Stability:
Three (3) Years from Manufacturing Date
Non-Toxicity:
ASO® was independently
tested by SGS USA and found to have no oral nor dermal toxicity*.
ASO® is completely non-toxic, biodegradable, non-caustic
and non-flammable. ASO® contains natural ingredients including
iodized sea salt and distilled water. It contains no microbiological
contaminants, no pesticides, is naturally biocidal and contains
no animal nor vegetable products or by-products.
SPECIFICATION RANGE
METHOD RESULT DETERMINATION
Specific Gravity: 1.00 to 1.06 Hydrometer 1.016 Passed
Density: 1.00 to
1.06 Digital Meter 1.016 Passed
pH: 0 to 14 Digital
Meter 8.46 Passed
Shelf Life: N/A Chamber
3 years Passed
A BRIEF EXPLANATION
OF THE ASO® MANUFACTURING PROCESS:
Materials: USP Food
Grade Sodium Chloride (CAS 7647-14-5)
Distilled water (CAS 7732-18-5)
Equipment: HDPE Processing Tank
Rare Earth Proprietary Anodes & Cathodes
Mixer
Preparation/Process:
Triple distilled water is transferred to HDPE processing tank
where the sodium chloride is introduced to the water. The distilled
water is then agitated by the mixer until all the salt is dissolved
into ionic form.
ASO® is manufactured
in an F.D.A. approved GMP laboratory designated for the manufacturing
of dietary supplements. The process involves the disassociation
of oxygen molecules from water molecules in an electrolytic
bath containing a mixture of water and sodium chloride. A regulated
current is then applied to two proprietary composite metal electrodes.
The hydrogen gas is released and the oxygen molecules are stabilized
in the electrolytic solution containing both the ASO® molecules
and trace minerals from the sodium chloride.
The ASO® is filtered
on-line into an HDPE food grade plastic drum. Inspection and
analysis is made for product conformance and quality. Once assured
that product meets standards, the container is mechanically
sealed and stored in a cool dry place.
GMO free:
NO GMO have been
used in any phase of the manufacturing process. ASO® Activated
Oxygen is a dietary supplement manufactured in the United States
of America. To the best of our knowledge, ASO® is not derived
from genetically modified starting raw materials, or additives
that are derived from genetically modified organisms, and do
not contain detectable levels of genetically modified materials,
(known as PCR negative). It is our understanding that the EU
labeling requirements are based on the presence of detectable
levels of transgenic material. Based on this information, this
product will not, on its own, require the labeling of these
foodstuffs and food ingredients as indicated in (EC) No. 1829/2003
and (EC) No. 1830/2003, (which came into law on the 7th of November,
2003) on the authorization, labeling, and traceability of genetically
modified organisms in food and feed. This product has not been
treated with ionizing radiations (gamma rays).
The chemical structure
formula for the ASO® molecule is:
Activated Oxygen
(ASO®) is exclusively manufactured for and distributed by
Connections Group, Inc. under the name of Berkana is laboratories
and manufacturing facilities that meet or exceed the United
States Food and Drug Administration (F.D.A.) and United States
Occupational and Safety Health Act (O.S.H.A.) standards. Further,
BERKANA warrantees that each sealed drum of ASO® has a three-year
shelf life from manufacturing date. ASO® was tested using
digital density/Specific Gravity Meters, Thermometers and Hydrometers
to ASTM Specifications and in accordance to ASTM Testing Methods.
*Copies of test reports are available upon written request.
ASO® does not consist, contain or is produced from GMOs
(genetically modified organisms).
MINERAL ANALYSIS
ASO®
Super Concentrate (35% Strength)
CONCENTRATION AMT
Per
ELEMENT (35% Strength)
5 mL Serv.
Aluminum mg/L 2.73
0.0098
Antimony mg/L <0.07
0.00025
Arsenic mg/L <0.04
0.00015
Barium mg/L 0.028
0.0001
Beryllium mg/L 0.0014
0.000005
Bismuth mg/L 0.028
0.0001
Boron mg/L 0.04 0.00015
Bromine mg/L <0.07
0.00025
Cadmium mg/L <0.007
0.000025
Calcium mg/L 11.06
0.0395
Carbon mg/L 518 1.85
Cerium mg/L <0.056
0.0002
Cesium mg/L <0.028
0.0001
Chloride mg/L 53,424.0079
79.5
Chromium mg/L 0.126
0.00045
Cobalt mg/L <0.04
0.00015
Copper mg/L 0.0112
0.00004
Dysprosium mg/L <0.014
0.00005
Erbium mg/L <0.04
0.00015
Europium mg/L <0.04
0.00015
Fluoride mg/L 0.112
0.0004
Gadolinium mg/L <0.028
0.0001
Gallium mg/L <0.04
0.00015
Germanium mg/L 0.04
0.00015
Gold mg/L <0.028
0.0001
Hafnium mg/L <0.014
0.00005
Holmium mg/L <0.014
0.00005
Indium mg/L <0.04
0.00015
Iodine mg/L 0.154
0.00055
Iridium mg/L <0.028
0.0001
Iron mg/L 0.126 0.00045
Lanthanum mg/L <0.028
0.0001
Lead mg/L <0.028
0.0001
Lithium mg/L 0.15
0.00075
Lutetium mg/L <0.042
0.00015
Magnesium mg/L 39.2
0.140
Manganese mg/L <0.03
0.00003
Mercury
mg/L <0.001 0.000001
CONCENTRATION AMT
Per
ELEMENT (35% Strength)
5 mL Serv.
Molybdenum mg/L <0.028
0.0001
Neodymium mg/L <0.014
0.00005
Nickel mg/L <0.028
0.00010
Niobium mg/L 0.04
0.00015
Osmium mg/L <0.028
0.0001
Palladium mg/L <0.028
0.0001
Phosphorus mg/L 36.4
0.13
Platinum mg/L <0.04
0.00015
Potassium mg/L 53.2
0.19
Praseodymium mg/L
<0.056 0.00020
Rhenium mg/L <0.014
0.00005
Rhodium mg/L <0.014
0.00005
Rubidium mg/L 0.028
0.0001
Ruthenium mg/L <0.028
0.0001
Samarium mg/L <0.014
0.00005
Scandium mg/L <0.04
0.00015
Selenium mg/L <0.056
0.0002
Silicon mg/L 13.58
0.0485
Silver mg/L <0.04
0.00015
Sodium mg/L 8,960.00
32.0
Strontium mg/L 0.266
0.00095
Sulfur mg/L 19.46
0.0695
Tantalum mg/L 0.028
0.0001
Tellurium mg/L <0.028
0.0001
Terbium mg/L 0.028
0.0001
Thallium mg/L 0.056
0.0002
Thorium mg/L <0.028
0.0001
Thulium mg/L <0.014
0.00005
Tin mg/L 0.028 0.0001
Titanium mg/L <0.007
0.000025
Tungsten mg/L <0.112
0.0004
Vanadium mg/L 0.014
0.00005
Ytterbium mg/L <0.0042
0.000015
Yttrium mg/L 0.004
0.00002
Zinc mg/L 0.49 0.00175
Zirconium mg/L 0.056
0.0002
TOTAL 31,915.550
31.91693
MINERALS
IN MG
MICROBIOLOGICAL ANALYSIS
ASO® Super Concentrate
(35% Strength)
ASO®
has been shown to be an effective anti-microbial agent pursuant
to extensive independent testing. The summary of results of
said testing indicate the following:
Use Dilution Method
955.14
Disinfectant Efficacy
Using
5% Strength ASO®
Staphylococcus aureus
ATCC 6538
Dilution 1:60 5
MIN + 10 MIN 0 15 MIN 0
Salmonella choleraesius
ATCC 10708
Dilution 1:90 5
MIN + 10 MIN 0 15 MIN 0
Pseudomonas aeruginosa
ATCC 15442
Dilution 1:80 5 MIN + 10 MIN + 15 MIN 0
+ = GROWTH
0
= NO GROWTH
Modified Antimicrobial
Preservative Effectiveness Test
Using 5% Strength
ASO®
(Dilution 1:1)
Staphylococcus aureus
ATCC 6538 0 HR > 99.9% 24 HR > 99.9%
Pseudomonas aeruginosa
ATCC 9027 0 HR > 99.9% 24 HR > 99.9%
Escherichia coli
ATCC 8739 0 HR >99.9% 24 HR > 99.9%
Candida albicans
ATCC 10231 0 HR > 99.9% 24 HR > 99.9%
Aspergillus niger
ATCC 16404 0 HR 99.5% 24 HR > 99.9%
Aspergillus
flavus ATCC 9643 0 HR 99.8% 24 HR > 99.9%
Minimum Inhibitory
Concentration
Using 25% Strength
ASO®
24 hour Test Incubation
60% Dilution 80%
Dilution
Escherichia coli
0157:H7 ATCC 43985 NO NO
Pseudomonas aeruginosa
ATCC 15442 NO YES
Salmonella choleraesuis
ATCC 10708 NO NO
Staphyloccus aureus
ATCC 6538 NO NO
NO = NO GROWTH AT
THE DILUTION RATE
YES = GROWTH AT THE
DILUTION RATE
Reference: SGS USA,
Inc. and Nelson Laboratories. Test reports available upon written
request.
Note: The above test
was conducted on 25% ASO®. 35% ASO® is 140% stronger
and similar, or better, results would be expected.
SHELF LIFE (Stability)
ASO® Activated Oxygen
Formation of Hydrogen Peroxide (H2O2) And/Or Chlorine Dioxide
(ClO2)
ASO® Accelerated
Standard Shelf Life testing for food (nutritional) supplements
Month Color Taste Odor Form Particulates ASO® mg/L % Change
pH
1 Clear Salty Halogen
Liquid None 350,000 0 7.4
6 Clear Salty Halogen
Liquid None 350,000 0 7.4
12 Clear Salty Halogen
Liquid None 350,000 0 7.4
18 Clear Salty Halogen
Liquid None 350,000 0 7.4
24 Clear Salty Halogen
Liquid None 350,000 0 7.4
Product samples maintained
at the following base temperatures of:
67º F (20º
C) ± 1.75º @ 50% humidity
77º F (25º
C) ± 1.75º @ 60% humidity
104º
F (40º C) ± 1.75º @ 75% humidity
Note that there were
no statistically significant data in any of the three samples
regardless of temperature or humidity differences.
LaMotte
Instrument Co. HP40 Code 3188 and Lamotte Instrument Co. Model
EDO Code 7414 Dissolved Oxygen Test Kits. Source: Lamotte Instrument
Co., Chestertown, MD 21620.
ClO2 and/or H2O2
Formation:
Accelerated Standard
Shelf Life testing for food (nutritional) supplements
Month ClO2 mg/L % Change H2O2 mg/L % Change
1 0 0 0 0
6 0 0 0 0
12 0 0 0 0
18 0 0 0 0
24 0 0 0 0
Note that there was
no formation of either Hydrogen Peroxide (H2O2) nor Chlorine
Dioxide (ClO2) during the test period in the solution of ASO®.
For H2O2: LaMotte
Instrument Co. HP40 Code 3188 and Lamotte Instrument Co. Model
EDO Code 7414 Dissolved Oxygen Test Kits.
For ClO2: LaMotte
Instrument Co. Model 3622 OctaSlide and Lamotte Instrument Co.
Model 3002 Chlorine Dioxide Insta Test Strips Test Kits.
Source: Lamotte Instrument
Co., Chestertown, MD 21620.
The above information
is provided for reference purposes only by BERKANA. All products
meet or exceed minimum quality control and ingredient standards
established by the U.S. Food and Drug Administration governing
the manufacture and sale for distribution of dietary supplements
and health and beauty products.
Shelf life refers
to the average amount of time ASO® may be stored without
nutritional or organoleptic (sensory) deterioration. A food
product can deteriorate for several reasons: aging, microbiological
decay, chemical and physical degradation, texture changes, etc.
These modes of deterioration are affected by many factors, both
intrinsic (of or within the product) and extrinsic (environmental
or outside the product). Controlling processing, packaging,
handling and storing of the product can slow deterioration of
food products. BERKANA’s expiration safety date refers
to the length of time ASO® can be expected to last without
significant deterioration.
The dates assigned
to ASO® are based on intrinsic and extrinsic factors, and
determined by systematic study or empirical data. Systematic
studies are designed to simulate actual or potential storage
and handling practices of a given product and estimate shelf
life through the course of chemical and analytical testing at
specific intervals throughout the expected shelf life. Systematic
studies may also rely on accelerated testing when dealing with
ASO® with expected long shelf lives. In some cases, shelf
life evaluations can be made based on existing information,
or empirical data.
Primary shelf life
tests conducted on ASO® have been conducted by independent
laboratories on standard test microorganisms. These reports
are available, upon request, from BERKANA.
Note that neither
Hydrogen Peroxide not Chlorine Dioxide is approved for human
consumption as a dietary supplement or as a food additive by
the U.S.F.D.A. The test data described above does not, in any
way, or in any manner, recommend the use of Hydrogen Peroxide
or Chlorine Dioxide for human consumption. This information
is only proved for product stability comparisons.
F.D.A. Recognition
of A.S.O.®
ASO® falls under
and meets all of the U.S.A. D.S.H.E.A. (Dietary and Supplement
Health Education Act) regulations enacted in 1997 for dietary
supplements. ASO® and its supporting literature is not intended
to diagnose, treat, cure or prevent any diseases or physiological
conditions. Individuals taking medications or under the are
of physicians should always let their health care providers
know that they are taking dietary supplements and should consult
those providers before taking any dietary supplements.
Ms. Barbara Schneeman,
Director of The United States Food and Drug Administration,
Department of Health and Human Services, Office of Nutritional
products, Labeling and Dietary Supplements, Center for Food
Safety and Applied Nutrition, pursuant to the provision of Rule
44 of the Federal Rules of Civil Procedure, and Title 42, United
States Code, Section 3505, and 21 CFR 5.22, has issued Certificate
of Free Sale F00099418 for “ASO Therapeutic Formula”.
Said Certificate of Free Sale is available upon written request.
ASO® is sold
under the following trade names:
Jozzer Hyper Oxygen
Drops ASO
AO2™ Activated
Stabilized Oxygen
O4+™ Stabilized
Oxygen
Executed this 5th
day of June 2006
